Lab at posters.

Lab Research Interest

The Comstock lab investigates fundamental physical processes in biology using advanced, precision single-molecule measurement and manipulation techniques. We observe in real-time individual protein molecular machines marching down DNA strands or ripping open RNA. We manipulate live cells and measure individual cellular electrochemistry. To do this we design and build frontier biophysical instrumentation combining optical tweezers and single-molecule fluorescence microscopy. We strive to watch biology in action without the obscuring effects of traditional ensemble methods.

We are a young interdisciplinary lab composed of scientists with a diversity of backgrounds. Students have the opportunity to participate in all aspects of biophysical research including: wet lab development and production of biological systems, design and construction of instrumentation (optics, electronics, software etc.), quantitative data analysis and modeling and collegial discussion. We are always on the lookout for excited new colleagues. Interested students and postdocs with a background in physics, biology, chemistry, or a related field are welcome to contact Prof. Comstock.


Mtr4p unwinding RNA cartoon.

November 21, 2016 Our work on the nuclear RNA helicase Mtr4p and TRAMP has been published in Nature Chemical Biology!

E. M. Patrick, Sukanya Srinivasan, Eckhard Jankowsky, and M. J. Comstock. “The RNA Helicase Mtr4p is a Duplex Sensing Translocase.” Nature Chemical Biology, 13, 99 (2017).

The work was led by our postdoc, Eric Patrick, in collaboration with Eckhard Jankowsky's lab at Case Western. We have directly shown that this helicase is a slow translocase that stalls without an upstream duplex to unwind.

NSF logo

September 1, 2015 Our technique has been featured again in Genetic Engineering and Biotechnology News (GEN): More Dynamic Protein Profiling. See on page 2: Single Molecule Nanometry.

June 28, 2015 We have received our first extramual funding! We have received a regular NSF award for "Investigation of RNA-Processing Protein Dynamics with Simultaneous High-Resolution Optical Traps and Single-Molecule Fluorescence" (MCB-1514706).

Fleezers measurement of helicase cartoon.

April 17, 2015 Matt's postdoctoral work (Chemla and Ha labs) is published in Science!

M.J. Comstock, K.D. Whitley, H. Jia, J. Sokoloski, T.M. Lohman, Taekjip Ha, and Y.R. Chemla. “Direct observation of structure-function relationship in a nucleic acid-processing enzyme,” Science, 348, 352 (2015).

Here we directly observe the dependence of helicase unwinding activity on protein stoichiometry and conformation.


We are presently supported by the National Science Foundation (MCB-1514706), MSU startup funds and the Cowen Endowed Chair which was made possible by the generous support of Randy Cowen and his family.


Matt Comstock, Department of Physics and Astronomy, Michigan State University

Biomedical Physical Sciences, 567 Wilson Road, Room 4216