Building a Microfluidic Mixer
These chips are designed to be fabricated from silicon (for visible fluorescence only) or fused silica (for UV fluorescence or UV-vis absorption).  Most steps of fabrication can be performed in any large clean room facility but to etch fused silica requires an ULVAC NLD570 Oxide etcher.  We use the etcher at UCLA Nanolab. 

Mask Designs (.tdb format requires L-edit, .dxf format requires Autocad)
T-mixer (tbd), T-mixer (dxf)
Serpentine Mixer (tdb), Serpentine Mixer (dxf)

Fabrication
Fused silica fabrication summary
Fused Silica fabrication protocol
Fused Silica bonding protocol
Silicon fabrication and bonding protocol

Mixer Manifold Designs (.SLDPRT format requires SolidWorks)
T-mixer pressure manifold summary
T-mixer manifold (pdf), T-mixer manifold (SLDPRT), in 3D
Serpentine mixer pressure manifold (pdf)

Mixer flow rate spreadsheets (MS Excel format)
T-mixer
Serpentine Mixer